Three people have been charged over an Extinction Rebellion protest where activists climbed on top of a train.
A man glued himself to a Docklands Light Railway (DLR) train carriage in Canary Wharf while a man and woman were removed from the roof on Wednesday.
Two men and a woman denied obstructing trains or carriages on the railway and were remanded in custody until 16 May.
Police have made further arrests but activists continue to block traffic at four sites around London.
Cathy Eastburn, 51, from Lambeth in south London, Mark Ovland, 35 of Somerton in Somerset and Luke Watson, 29, of Manuden in Essex, appeared before Highbury Magistrates’ Court and pleaded not guilty to the charge.
They will next appear at Blackfriars Crown Court on 16 May.
British Transport Police said it “continues to deploy additional officers throughout the London rail network to deter and disrupt further protest activity”.
More than 425 have been arrested since the protests began on Monday.
Activists are continuing to block traffic at four sites in the capital despite police making more than 100 arrests on Wednesday.
Marble Arch, Parliament Square, Oxford Circus and Waterloo Bridge have been occupied by protesters since Monday.
Transport for London warned delays around those areas were expected “throughout the day”.
Footage posted on social media of police officers dancing with activist at Oxford Circus on Wednesday evening has been condemned as “unacceptable behaviour” by the Met.
“We expect our officers to engage with protestors but clearly their actions fall short of the tone of the policing operation,” added Cdr Jane Connors.
Some protesters have been seen returning to the blockades despite being arrested.
Police action to deter activists was having the “opposite” effect, according to environmental scientist Dominic Goetz who has returned to Waterloo Bridge following his arrest on Tuesday.
“I don’t know whether I will be arrested again or not. If I am, I think the consequences will probably not be particularly severe,” the 47-year-old said.
A group of demonstrators has been blocking Vauxhall Bridge for short periods of time as part of a “swarming” protest.
Similar intermittent roadblocks have also been formed by activists at Piccadilly Circus.
Extinction Rebellion co-founder Dr Gail Bradbrook has warned that the group’s tactics could escalate “if our demands are not met”.
She said: “More people are joining us all the time. We’re having a fantastic time here.”
Ken Marsh, chairman of the Met Police Federation, said it was “very difficult” for police to deal with the activists as “we have never dealt with something like this before”.
What is Extinction Rebellion?
Since the group was set up last year, members have shut bridges, poured buckets of fake blood outside Downing Street, blockaded the BBC and stripped semi-naked in Parliament.
It has three core demands: for the government to “tell the truth about climate change”; to reduce carbon emissions to zero by 2025; and to create a citizens’ assembly to oversee progress.
Controversially, the group is trying to get as many people arrested as possible.
But critics say they cause unnecessary disruption and waste police time when forces are already overstretched.